Research Interests
My research interest centers on mucosal immunology. Inflammatory bowel disease (IBD) is a disease of chronic inflammatory condition of the gastrointestinal tract. The causes of human IBD remain unknown. We show for the first time that anti-CXCL10 Ab treatment abrogates colitis in IL-10-/- mice. Understanding the steps in leukocyte migration and activation, and how CXCL10 mediates the host immune response during the progression of inflammatory bowel disease, will help better understand this disease and other inflammatory conditions. Recently I have started a similar project on poorly regulated immune responses to Mycobacterium avium subspecies paratuberculosis, which we are using to induce chronic colitis in IL-10-/- mice. It is my sincere hope that my work will contribute to a better understanding of disease pathogenesis and the development of vaccines as well as inflammation therapeutics in general.
I wish to continue my work in the field of mucosal immunology and inflammation related to chemokine and alternative medicine to treat inflammation. I have found that CXCL10 expressing CD4+ T cells, NK cells, neutrophils and macrophages mediate colitis in IL-10-/- mice by inducing the chemotaxis of leukocytes and activation of dendritic cells to and from the intestinal lamina propria and mesenteric lymph nodes, where they expand reactive Th1 cells. I will continue to dissect the mechanisms of CXCR3 ligand expression by mucosal and CD4+ T cells, NK cells and dendritic cells that are essential for the induction and maintenance of colitis mediated by CXCR3+ Th1 cells.
Recent Publications
Singh U.P., Singh, S., Singh, R., Cong, Y., Taub, D.D. and J.W. Lillard, Jr. (2008) Chronic colitis and CXCL10-producing mucosal T helper, NK and NKT cells in IL10-/- mice can be abrogated by CXCL10 blockade. Journal of Interferon & Cytokine Research. 28(1): 31-43.
Singh U.P., A. Krishna, and Kunwar P. Bhatnagar (2008) Changes in serum leptin, insulin, androstenedione, and luteinizing hormone during ovarian cycle in the bat, Taphozous longimanus. Acta Biologica Hungarica. 59: 1-16.
Singh U.P., A. Krishna, and Kunwar P. Bhatnagar (2007) Year round plasma leptin and androgen concentrations in a tropical bat. Acta Theriologica. 52(2): 129-140.
Singh U.P., Singh S, Chandrasekar Venkataraman and James W. Lillard, Jr. (2007) CXCR3 Axis: Role in Inflammatory Bowel Disease and its Therapeutic Implication. Endocrine, Metabolic & Immune Disorder: Drug Target. 7(2): 111-123.
Singh U.P., Singh S, Singh R, Frederick D. Quinn, Morris E. Potter & James W. Lillard, Jr. (2007) Influence of Mycobacterium avium paratuberculosis on colitis development and specifics immune responses during disease. Infection and Immunity. 75 (8): 3722-3278.
Kohsaka, T., Singh U.P., Yogo, K., Sasada, H., Taya, K., Hashizume, K. (2007) Expression and cellular pattern of Relexin mRNA in porcine corpora lutea during pregnancy. Cell Tissue Research. 330(2): 303-312.
Ravichandran Palaniappan, Shailesh Singh, Singh U.P., Edwin W. Ades, David E. Briles, Susan K. Hollingshead, Walter Royal, III, Jacquelyn S. Sampson, Jonathan K. Stiles, Dennis D. Taub, and James W. Lillard, Jr. (2006) CCL5 Modulates Pneumococcal Immunity and Carriage. Journal of Immunology. 176: 2346-2356.
Singh U.P., A. Krishna, Timothy D. Smith and Kunwar P. Bhatnagar (2005) Histochemical localization of enzymes and lipids in the ovary of a Vespertilionid, bat, Scotophilus heathi, during reproductive cycle. Brazilian Journal of Biology. 65(1): 179-186.
Palaniappan R, Singh S, Singh U.P., Sakthivel SK, Ades EW, Briles DE, Hollingshead SK, Paton JC, Sampson JS, and J.W. Lillard, Jr. (2005) Differential PsaA-, PspA-, PspC-, and PdB-specific immune responses in a mouse model of Pneumococcal Carriage. Infection Immunity. 73(2): 1006-13.
Singh U.P., S. Singh, R. Palaniappan, D.D. Taub, and J.W. Lillard, Jr. (2004) vMIP-II versus I-309 effects on adaptive immunity. Journal of Immunology. 173:5509-5516.
Singh U.P., Singh S., Boyaka, P.N., McGhee, J.R., and Lillard, J.W. Jr. (2004) Granulocyte chemotactic protein-2 mediates adaptive immunity through CXCR1 interactions and CD28/B7 up regulation. Journal of Leukocyte Biology. 76:1240-1247.
S. Singh, U.P. Singh, W.E. Grizzle, and J.W. Lillard, Jr. (2004) CXCL12-CXCR4 interactions modulate prostate cancer cell migration, metalloproteinase expression and invasion. Laboratory Investigation. 84: 1666-1676.
S. Singh, U.P. Singh, Jonathan, K. Stiles, W.E. Grizzle, and J.W. Lillard, Jr. (2004) Expression and functional role of CCR9 in prostate cancer cell migration and invasion. Clinical Cancer Research. 10 (24): 8743-50.
Lillard, Jr., J. W., U.P. Singh, P.N. Boyaka, S. Singh, D.D. Taub and J.R. McGhee (2003) MIP-1alpha and MIP-1beta differentially mediate mucosal and systemic adaptive immunity. Blood. 101: 807-814.
Singh U.P., S. Singh, D.D. Taub, and J.W. Lillard, Jr. (2003) Inhibition of IP-10 abrogates colitis in IL-10-/- mice. Journal of Immunology. 171:1401-1406.
Singh U.P., S. Singh, D.D. Taub, and J.W. Lillard, Jr. (2003) Interferon-inducible chemokines differentially mediate adaptive immunity and colitis. Journal of Interferon & Cytokine Research. 23(10): 591-600.
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